Laboratory Animal Back

Animal House

The Animal House Facility maintains thirty one strains of mice (inbred, outbred, transgenic, knock-out & immune deficient ) , three strains of rats, two strains of hamsters, three strains of rabbits and two strains of guinea pigs in different rooms. The average number of animals at any one time is about 6000. The animal rooms are maintained at 22.2ºC with a relative humidity of 55.5%. A 12-hour light-dark cycle is maintained with automatic timers. All the environmental factors are moderated by a computerized central monitoring system. The animal facility is registered under CPCSEA, Government of India. 

The main objective of the Animal House is to supply defined strains of mice, rats, guinea pigs, hamsters and rabbits to the scientific community of the Centre. The propagation of all defined strains is done in two-tier system, i.e., foundation colony (SPF colony) and production colony.

Mouse genotype laboratory was recently established with equipment related to genetic monitoring and biochemical monitoring of laboratory animals. Routine check for genetic contamination in lab animals is carried out using nine microsatellite SSLP markers (D1 Mit 21,D4 Mit 53, D5 Mit 18, D4 Mit 7, D15 Mit 8, D16 Mit 4, D14 Mit 40, D19 Mit 41 and D2Mit61) and ten biochemical markers ( Hbb, Car- I, Car- II, Gpi- I, Est- I, Est- III, Mod- I, Idh- I, Pgm- I and Trf- I ) using PCR and enzyme markers analyzed through cellulose acetate electrophoresis techniques.

The Animal House team comprises of 18 staff including 11 qualified animal caretakers, who have undergone specialized training in breeding and management of laboratory animals course offered by the Indian Council of Medical Research at the Laboratory Animal Information Service Centre, Hyderabad. 

A trained veterinarian carries out regular health monitoring of all animals to ensure that they are free from contagious diseases. To prevent the transmission of infection, standard hygienic procedures, i.e., use of sterilized bedding, autoclaved cages, zero  bacteria/autoclaved drinking water and handling of animals using gloved hands are followed. Necropsy and autopsy of suspected contagious/infectious animals are performed by a veterinarian and prophylactic measures are implemented.

Regular screening of microorganisms in the SPF and production colony mice, feed, water, paddy husk, and isolators are carried out by various microbiological and pathological techniques, viz., culture, differential staining techniques, biochemical tests and histopathology. All animal house records are updated and maintained everyday, assisted by unique software.

Mice strains available

S.No Strain Name Human disease model Source
1 B6.129S4-Icam1tm1Jcgr/J
(Stock No: 002867)
Cardiovascular disorder Jackson Laboratory, USA
2. B6.129P2-Apoetm1Unc/J
(Stock No: 002052 )
Cardiovascular and neurological disorders Jackson Laboratory, USA
3 MMTV Cre (Mouse mammary tumour virus) useful for creation of conditional BRCA1 knockout mice- breast cancer National cancer Institute, USA
4 B6.129S2-Trp53tm1Tyj/J
(Stock No: 002101 )
Cancer research Jackson Laboratory
5 NOD/LtJ
(Stock No:001976)
Type I diabetes insulin-dependent diabetes mellitus Jackson Laboratory, USA
6 B6.BKS-Leprdb (Leprdb/Leprdb)
(Stock No: 000697)
Type I diabetes Jackson Laboratory, USA
7 B6C3Tg(APPswee PSEN1dE9)85 Dbo/J
(Stock No: 004462)
Alzheimer’s disease model Jackson Laboratory, USA
8 Pitx3ak/2 J
(Stock NO: 000942)
Parkinson disease model Jackson Laboratory, USA
9 129-Achetm1Loc/J
(Stock No: 005987)
Parkinson disease model Jackson Laboratory, USA
10 APC Cre/ APC flx Colon cancer Cardiff University, UK
11 BAP (BRCA 1 -/ - / p53 -/- ) Breast Cancer Cardiff University, UK
12 IP6K1 mice Inositol hexaphosphate kinase 1 John Hopkins University, USA
13 CD-1 FOX n1nude Immunodeficient mice( Deficient in T lymphocytes ) Charles River, USA
14 NOD. CB17-Prkdc <scid>/J CBySmn.CB17-Prkdcscid/J
( Stock No: 001303 )
Deficiency of B & T lymphocytes (severe combined immuno deficient mice) Jackson Laboratory, USA
15 Balb C/J
(Stock No: 000651)
Inbred mice Jackson Laboratory
16 CBA/ca J
(Stock No: 000654)
Inbred mice Jackson Laboratory
17 129 Xl/ SvJ
(Stock number :000691)
Inbred mice Jackson Laboratory
18 Neo r Neomycin resistance National Institute of Immunology, Delhi
19 RIII mice Inbred mice Jackson Laboratory, USA
20 Y del Inbred mice Jackson Laboratory, USA
21 C57BL 6/J
(Stock No: 000664)
Inbred mice Jackson Laboratory, USA
22 C57BL 6 /N crl Inbred mice Charles River, USA
23 CD-1 Out bred mice Charles River, USA
24 129-Alp1<tm1(cre)Nagy. Stock number : 008569 Inbred mice Jackson Laboratory, USA
25 Dicer 1< tm1Bdh>
(Stock No: 006001)
Inbred mice Jackson Laboratory, USA
26 OG2tg (OCT4GFP) Inbred mice VCCRI, Australia
27 B6.129S4-Gt(ROSA)26Sortm1(FLP1)Dym/RainJ
( Stock No : 009086 )
Targeted Mutation Jackson Laboratory, USA
28 B6;129-Psen1tm1Mpm Tg(APPSwe,tauP301L)1Lfa/Mmjax Alzheimer's disease        MMRRC, USA
29 B6.Cg-Tg(SOD1*G37R) 29Dpr/J  Amyotrophic Lateral Sclerosis (ALS) Jackson Lab,USA
30 C57BL/6J-Tg(Th-SNCA*A30P*A53T)39Eric/J Parkinson Disease Jackson Lab, USA

Other animals

S.No Strain Name Human disease model Source
31 Wistar Rats Out bred National Institute of Nutrition, Hyderabad
32 Sprague Dawley Rats Out bred National Institute of Nutrition, Hyderabad
33 Newzeland white Rabbits Out bred National Institute of Nutrition, Hyderabad
34 Golden Syrian Hamster Out bred National Institute of Nutrition, Hyderabad
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