1. Paila, Y.D., Tiwari, S., and Chattopadhyay, A. (2009) “Are Specific Nonannular Cholesterol Binding Sites Present in G-protein Coupled Receptors? Biochim. Biophys. Acta (Biomembranes) 1788: 295-302.

2. Ganguly, S., Pucadyil, T.J., and Chattopadhyay, A. (2008) “Actin Cytoskeleton Dependent Dynamics of the Serotonin1A Receptor Correlates with Receptor Signaling” Biophys. J. 95: 451-463.

3. Paila, Y.D., Murty, M.R.V.S., Vairamani, M., and Chattopadhyay, A. (2008) “Signaling by the Human Serotonin1A Receptor is Impaired in Cellular Model of Smith-Lemli-Opitz Syndrome” Biochim. Biophys. Acta (Biomembranes) 1778: 1508-1516.

4. Shrivastava, S., Paila, Y.D., Dutta, A., and Chattopadhyay, A. (2008) “Differential Effects of Cholesterol and its Immediate Biosynthetic Precursors on Membrane Organization” Biochemistry 47: 5668-5677.

5. Haldar, S., and Chattopadhyay, A. (2007) “Dipolar Relaxation within the Protein Matrix of the Green Fluorescent Protein: A Red Edge Excitation Shift Study” J. Phys. Chem. B 111: 14436-14439.

6. Pucadyil, T.J., and Chattopadhyay, A. (2007) "Cholesterol Depletion Induces Dynamic Confinement of the G-protein Coupled Serotonin1A Receptor in the Plasma Membrane of Living Cells" Biochim. Biophys. Acta (Biomembranes) 1768: 655-668.

7. Raghuraman, H., and Chattopadhyay, A. (2007) "Orientation and Dynamics of Melittin in Membranes of Varying Composition utilizing NBD fluorescence" Biophys. J. 92: 1271-1283.

8. Pucadyil, T.J., and Chattopadhyay, A. (2007) "Cholesterol: A Potential Therapeutic Target in Leishmanial Infection ?" Trends Parasitol. 23: 49-53.

9. Rawat, S.S., Kelkar, D.A., and Chattopadhyay, A. (2005) "Effect of Structural Transition of the Host Assembly on Dynamics of an Ion Channel Peptide: A Fluorescence Approach" Biophys. J. 89: 3049-3058.

10. Kelkar, D.A., and Chattopadhyay, A. (2005) "Effect of Graded Hydration on the Dynamics of an Ion Channel Peptide: A Fluorescence Approach" Biophys. J. 88: 1070-1080.

11. Pucadyil, T.J., Kalipatnapu, S., Harikumar, K.G., Rangaraj, N., Karnik, S.S., and Chattopadhyay, A. (2004) "G-protein-dependent Cell Surface Dynamics of the Human Serotonin1A Receptor Tagged to Yellow Fluorescent Protein" Biochemistry 43: 15852-15862.

12. Raghuraman, H., and Chattopadhyay, A. (2004) "Interaction of Melittin with Membrane Cholesterol: A Fluorescence Approach" Biophys. J. 87: 2419-2432.

13. Kalipatnapu, S., and Chattopadhyay, A. (2004) "A GFP Fluorescence-based Approach to Determine Detergent Insolubility of the Human Serotonin1A Receptor" FEBS Lett. 576: 455-460.

14. Rawat, S.S., Kelkar, D.A., and Chattopadhyay, A. (2004) "Monitoring Gramicidin Conformations in Membranes: A Fluorescence Approach" Biophys. J. 87: 831-843.

15. Pucadyil, T.J., and Chattopadhyay, A. (2004) "Cholesterol Modulates Ligand Binding and G-protein Coupling to Serotonin1A Receptors from Bovine Hippocampus" Biochim. Biophys. Acta (Biomembranes) 1663: 188-200.

16. Pucadyil, T.J., Tewary, P., Madhubala, R., and Chattopadhyay, A. (2004) "Cholesterol is Required for Leishmania donovani Infection: Implications in Leishmaniasis" Mol. Biochem. Parasitol. 133: 145-152.

17. Chattopadhyay, A., Rawat, S.S., Kelkar, D.A., Ray, S., and Chakrabarti, A. (2003) "Organization and Dynamics of Tryptophan Residues in Erythroid Spectrin: Novel Structural Features of Denatured Spectrin Revealed by the Wavelength-Selective Fluorescence Approach" Protein Sci. 12: 2389-2403.

18. Chattopadhyay, A., Harikumar, K.G., and Kalipatnapu, S. (2002) "Solubilization of High Affinity G-Protein-Coupled Coupled Serotonin1A Receptors from Bovine Hippocampus using Pre-Micellar CHAPS at Low Concentration" Mol. Membr. Biol. 19: 211-220.

19. Rukmini, R., Rawat, S.S., Biswas, S.C., and Chattopadhyay, A. (2001) "Cholesterol Organization in Membranes at Low Concentrations: Effects of Curvature Stress and Membrane Thickness" Biophys. J. 81: 2122-2134.

20. Harikumar, K.G., and Chattopadhyay, A. (1999) "Differential Discrimination of G-Protein-Coupling of Coupled Serotonin1A Receptors from Bovine Hippocampus by an Agonist and Antagonist" FEBS Lett. 457: 389-392.

21. Chattopadhyay, A., and Mukherjee, S. (1999) "Depth-Dependent Solvent Relaxation in Membranes: Wavelength-Selective Fluorescence as a Membrane Dipstick" Langmuir 15: 2142-2148.

22. Mukherjee, S., and Chattopadhyay, A. (1996) "Membrane Organization at Low Cholesterol Concentrations: A Study using 7-Nitrobenz-2-oxa-1,3-diazol-4-yl-Labeled Cholesterol" Biochemistry 35: 1311-1322.

23. Chattopadhyay, A., and Mukherjee, S. (1993) "Fluorophore Environments in Membrane?Bound Probes: A Red Edge Excitation Shift Study" Biochemistry 32: 3804-3811.

24. Chattopadhyay, A., and McNamee, M.G. (1991) "Average Membrane Penetration Depth of Tryptophan Residues of the Nicotinic Acetylcholine Receptor by the Parallax Method" Biochemistry 30: 7159-7164.

25. Chattopadhyay, A., and London, E. (1987) "Parallax Method for Direct Measurement of Membrane Penetration Depth utilizing Fluorescence Quenching by Spin-Labeled Phospholipids" Biochemistry 26: 39-45.

Membrane Organization and Dynamics using the Wavelength-Selective        Fluorescence Approach

 

 

Role of Membrane Cholesterol in Leishmaniasis

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

The major research interest of our group is centered on membrane and receptor biology using a variety  of  biophysical,  biochemical  and  cell biological approaches.  Fluorescence-based spectroscopic and microscopic approaches are extensively used in our laboratory.